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Chapter 12 Human Ovarian Tissue Slow Freezing.

Debra A Gook1

  • 1Reproductive Services/Melbourne IVF and Department of Obstetrics and Gynaecology, Royal Women's Hospital, University of Melbourne, Cnr Gratten Street and Flemington Road, Parkville, VIC, 3052, Australia. debra.gook@mivf.com.au.

Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2017
PubMed
Summary

Human ovarian tissue cryopreservation is a validated laboratory procedure. Successful twin births demonstrate the preservation of primordial follicles in cryopreserved ovarian tissue grafts.

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Area of Science:

  • Reproductive medicine
  • Cryobiology
  • Ovarian tissue transplantation

Background:

  • Ovarian tissue cryopreservation is increasingly adopted in assisted reproductive technology (ART) laboratories.
  • Validation of cryopreservation methods is crucial for clinical application.

Purpose of the Study:

  • To confirm the efficacy of ovarian tissue cryopreservation.
  • To demonstrate the long-term viability and functionality of cryopreserved ovarian tissue.

Main Methods:

  • Histological assessment of cryopreserved ovarian tissue.
  • Functional evaluation of cryopreserved ovarian tissue.
  • Grafting of cryopreserved ovarian tissue at a heterotopic site.

Main Results:

Keywords:
CortexCryopreservationDehydrationMedullaRehydration

Related Experiment Videos

  • The cryopreservation procedure has been validated at both histological and functional levels.
  • Unequivocal evidence of primordial follicle preservation was observed.
  • Successful twin births resulted from grafted cryopreserved ovarian tissue.

    • Conclusions:

    • Cryopreservation of human ovarian tissue is a reliable ART laboratory procedure.
    • The demonstrated success, including live births, supports the clinical use of ovarian tissue cryopreservation for fertility preservation.