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Appendix D: Irvine Scientific® Vitrification System.

Matthew VerMilyea1, Amber Brewer2

  • 1Ovation Fertility, 6500 N. Mopac Expy, Bldg 3 Suite 3102, Austin, Texas, 78731, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2017
PubMed
Summary

This study details the use of Irvine Scientific vitrification and warming solutions with the CryoTip device for human embryo and oocyte cryopreservation. The CryoTip offers a closed system for safe and effective vitrification, with successful pregnancy outcomes reported.

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Area of Science:

  • Reproductive medicine
  • Cryobiology
  • Embryology

Background:

  • Vitrification is a key technique for cryopreserving human gametes and embryos.
  • The CryoTip device offers a closed system for vitrification, minimizing contamination risks.
  • The Irvine Scientific Vit Kits provide necessary solutions for this process.

Purpose of the Study:

  • To describe the protocol for using Irvine Scientific vitrification solutions and the CryoTip device.
  • To highlight the safety and efficacy of the CryoTip for human embryo and oocyte cryopreservation.
  • To present evidence of successful pregnancies following CryoTip vitrification.

Main Methods:

  • Utilized Irvine Scientific vitrification and warming solutions (Vit Kits).
  • Employed the CryoTip vitrification device for rapid cooling of oocytes, cleavage stage embryos, and blastocysts.
Keywords:
CryoTip®Irvine ScientificVit Kits®embryo vitrification

Related Experiment Videos

  • Followed a detailed protocol for gamete and embryo handling within the closed CryoTip system.

    • Main Results:

    • Successful pregnancies have been reported using the CryoTip device for oocytes, cleavage stage embryos, and blastocysts.
    • The CryoTip, as a closed carrier, eliminates the risk of disease transmission via liquid nitrogen.
    • CryoTip has FDA clearance and CE mark approval, demonstrating its safety and reliability.
    • Results are comparable to other open system vitrification devices.
    • The device is also suitable for cryopreserving small sperm volumes.

    Conclusions:

    • The CryoTip device, used with Irvine Scientific solutions, is a safe, reliable, and effective method for human embryo and oocyte vitrification.
    • The closed system design enhances safety by preventing contamination.
    • This method supports successful cryopreservation and subsequent pregnancies.