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Improved ganoderic acids production in Ganoderma lucidum by wood decaying components.
Yanru Hu1,2,3,4, Shakeel Ahmed1,2,3,4, Jiawei Li1,2,3,4
1Laboratory of Drug Discovery and Molecular Engineering, Department of Medicinal Plants, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.
Scientific Reports
|April 20, 2017
Summary
Microcrystalline cellulose (MCC) and D-galactose significantly boost Ganoderic acid (GA) production in Ganoderma lucidum. These compounds enhance GA biosynthesis, offering a strategy for industrial production of this Traditional Chinese Medicine component.
Area of Science:
- Mycology
- Biotechnology
- Natural Product Chemistry
Background:
- Ganoderma lucidum is a revered Traditional Chinese Medicine (TCM).
- Ganoderic acids (GAs) are key bioactive compounds, primarily produced in the mushroom's fruiting body and primordium, with limited production in mycelia.
- The regulatory mechanisms governing GA biosynthesis, especially in submerged fermentation, remain largely unexplored.
Purpose of the Study:
- To identify effective inducers for Ganoderic acid (GA) production in Ganoderma lucidum during submerged liquid fermentation.
- To investigate the potential of wood-derived chemical components as GAs inducers.
- To elucidate the role of identified inducers in the biosynthesis pathway.
Main Methods:
- Screening of various wood-derived chemical components as potential inducers for GA production.
- Optimization of inducer concentrations and fermentation time-course.
- Analysis of enzyme transcription levels involved in GA biosynthesis.
Main Results:
- Microcrystalline cellulose (MCC) at 1.5% increased GAs production by 85.96%.
- D-galactose at 0.5% increased GAs production by 63.90%.
- MCC and D-galactose significantly induced the transcription of key enzymes in the GAs biosynthesis pathway, suggesting they act as signaling molecules rather than carbon sources.
Conclusions:
- Microcrystalline cellulose (MCC) and D-galactose are effective inducers for Ganoderma lucidum Ganoderic acid (GA) production in submerged fermentation.
- These compounds can be industrialized to enhance the yield of GAs, important compounds in Traditional Chinese Medicine.
- Further research into the signaling roles of MCC and D-galactose could optimize G. lucidum cultivation for secondary metabolite production.