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Can Ultrasound Imaging Predict the Success of an Experimental Steatofibrosis Model?
Rustu Turkay1, Abdurrahman Fatih Aydin, Ilknur Bingul
1*Radiology Department, Istanbul Bakirkoy Dr. Sadi Konuk Training and Research Hospital; †Department of Biochemistry, Faculty of Medicine, Istanbul University; ‡Department of Pathology, Faculty of Medicine, Yeditepe University; and §Department of Radiology, Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Abstract:
Our goal was to evaluate the role of ultrasound (US) imaging in an experimental 2-hit steatofibrosis rat model. Nineteen female Sprague-Dawley rats were divided into 2 groups: control group (n = 6) and high-fat diet carbontetrachloride (HFD-CCl4) group (n = 13) that was fed with HFD for 14 weeks. Ultrasound was performed to evaluate liver steatosis. The HFD-CCl4 group rats were divided further into 2 subgroups: HFD rats with liver steatosis [US (+) group; n = 6] and without steatosis [US (-) group; n = 7]. All rats in the subgroups were administered with CCl4. In both US (+) and US (-) subgroups, steatosis score, fibrosis score, triglyceride, and hydroxyproline contents were markedly higher compared with the control group. When compared with the US (-) group, triglyceride and hydroxyproline contents were significantly higher in the US (+) group, whereas steatosis and fibrosis scores were not different. Ultrasound imaging may be useful to assess the success of a 2-hit experimental steatofibrosis model.