Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Febrile neutropenia adversely affects chemomobilization outcomes in patients with multiple myeloma and increases risk for post-transplant infection in those with lymphoma: real-world multicenter data.

Blood science (Baltimore, Md.)·2026
Same author

Design and realization of a sputter deposition system for the <i>in situ</i> and <i>in operando</i> use in polarized neutron reflectometry experiments: Novel capabilities.

Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment·2026
Same author

Long-term complete remission and minimal side effects after CD19/CD22 CAR-T cell cocktail therapy in a living donor with DLBCL: A rare case report.

Cell transplantation·2026
Same author

Safety and Efficacy of Reduced-Intensity Conditioning Allogeneic Hematopoietic Stem Cell Transplantation for Salvage Therapy of Myelofibrosis.

Transplantation proceedings·2026
Same author

Long-term follow-up demonstrates the curative potential of dual CD19/CD22 CAR-T-cell therapy alone or combined with autologous stem cell transplantation in TP53-altered relapsed/refractory B-cell non-Hodgkin lymphoma.

Signal transduction and targeted therapy·2026
Same author

Comparative Analysis of T-Cell and Bone Marrow Chimerism for Relapse Prediction in Acute Leukaemia Post-Transplantation.

HLA·2026

Related Experiment Video

Updated: Mar 4, 2026

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

14.6K

Saline is a more appropriate solution for microvesicles for flow cytometric analyses.

Xing Xin1, Peiling Zhang1, Xing Fu1

  • 1Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China.

Oncotarget
|April 21, 2017
PubMed
Summary

Phosphate-buffered saline (PBS) can cause false positives in microvesicle (MV) detection via flow cytometry. Saline is a more suitable buffer for accurate MV quantification, avoiding artifactual vesicle formation.

Keywords:
flow cytometrymicrovesiclesphosphate-buffered salinesaline

More Related Videos

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.7K
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

24.1K

Related Experiment Videos

Last Updated: Mar 4, 2026

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

14.6K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.7K
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

24.1K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Cancer Research

Background:

  • Microvesicles (MVs) are crucial in cancer research, carrying molecular and oncogenic signatures.
  • Current methods for MV detection using flow cytometry may be affected by buffer choice.
  • Concerns exist regarding the accuracy of MV quantitation due to buffer-induced artifacts.

Purpose of the Study:

  • To investigate the impact of different buffers on microvesicle (MV) detection by flow cytometry.
  • To identify potential sources of false positive or negative results in MV quantitation.
  • To determine the most suitable buffer for accurate MV analysis.

Main Methods:

  • Microvesicles (MVs) were detected using flow cytometry.
  • Three different solutions were tested as buffers: water, saline, and phosphate-buffered saline (PBS).
  • Annexin V binding and quantification of MV markers (CD3, CD19) were assessed.

Main Results:

  • Phosphate-buffered saline (PBS) induced the formation of artifactual nano-sized vesicles when combined with annexin V binding buffer.
  • PBS significantly increased the Annexin V positive rate, leading to false positive results.
  • False negative results were observed for MV markers (CD3, CD19) when using PBS.
  • Saline and water did not produce similar artifactual events.

Conclusions:

  • Phosphate-buffered saline (PBS) is unsuitable for microvesicle (MV) quantitation by flow cytometry due to artifactual vesicle formation.
  • The precipitation of calcium phosphates from PBS and binding buffer likely causes these artifacts.
  • Saline is recommended as a more appropriate buffer for accurate MV detection and quantification, considering osmotic pressure.