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Related Experiment Videos

A rabbit Ig lambda L chain C region gene encoding C21 allotopes.

R M Duvoisin1, D J Hayzer, D Belin

  • 1Department of Medical Biochemistry, Faculty of Medicine, University Medical Center, Geneva, Switzerland.

Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

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Southern blot analysis reveals rabbit C lambda 5 gene encodes C21 allotypes. The C lambda 6 gene, present in all rabbits, does not encode C7 or C21 allotypes, indicating genetic differences in lambda chain allotype expression.

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Genomics

Background:

  • Rabbit immunoglobulin lambda light chains exhibit allotypic variations, including C7 and C21.
  • Understanding the genetic basis of these allotypes is crucial for immunoglobulin research.

Purpose of the Study:

  • To investigate the genomic organization of rabbit C lambda genes.
  • To determine which C lambda gene encodes the C21 allotype.

Main Methods:

  • Southern blot analysis of rabbit germ-line DNA.
  • Cloning and sequencing of C lambda region genes.
  • Gene expression studies using plasmid vectors and immunoprecipitation.

Main Results:

  • A 12-kbp EcoRI fragment, containing two C lambda genes (C lambda 5 and C lambda 6) with associated J lambda segments and recombination signals, was identified in rabbits expressing the C21 allotype.

Related Experiment Videos

  • The C lambda 5 gene, found on a 2-kbp BamHI fragment, is absent in rabbits lacking the C21 allotype.
  • The C lambda 6 gene, present in all rabbits, differs from C lambda 5 by 17 base positions.
  • Expression studies demonstrated that the C lambda 5 gene product is immunoprecipitated by anti-C21 alloantisera, confirming its role in C21 allotype expression.

    • Conclusions:

    • The C lambda 5 gene is responsible for encoding the C21 allotype in rabbits.
    • The C lambda 6 gene, while present in all rabbits, does not appear to encode the studied C7 or C21 allotypes.
    • This study elucidates the genetic basis of specific rabbit lambda chain allotypes.