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TRPA1 expression and its functional activation in rodent cortex.

Ehsan Kheradpezhouh^1,2, Julian M C Choy³, Vincent R Daria³

¹Eccles Institute of Neuroscience, John Curtin School of Medical Research, Australian National University Node, Acton, Australian Capital Territory 2601, Australia ehsan.kheradpezhouh@anu.edu.au.

|April 21, 2017

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This summary is machine-generated.

Transient Receptor Potential Ankyrin 1 (TRPA1) channels are present in the rodent somatosensory cortex. TRPA1 activation modulates layer 5 pyramidal neuron activity, offering a new method to study its function.

Area of Science:

Neuroscience
Molecular Biology
Physiology

Background:

Transient Receptor Potential Ankyrin 1 (TRPA1) channels are known for roles in pain and inflammation.
The presence and function of TRPA1 in the mammalian cortex are not well understood.

Purpose of the Study:

To investigate the expression and function of TRPA1 in the rodent somatosensory cortex.
To explore novel methods for studying TRPA1 activity in cortical neurons.

Main Methods:

Immunostaining to detect TRPA1 expression in rodent somatosensory cortex.
Whole-cell electrophysiology and Ca2+ imaging to assess neuronal activity.
Two-photon photoswitching using optovin to optically activate TRPA1.

Main Results:

Keywords:

cortex layer 5 pyramidal neurons optovin photoswitching trpa1

Related Experiment Videos

TRPA1 expression was confirmed in rodent somatosensory cortex layer 5 (L5) pyramidal neurons.
TRPA1 agonists increased intracellular Ca2+ and depolarized L5 neurons, effects blocked by antagonists and absent in knockout mice.
Optovin-mediated optical activation of TRPA1 induced neuronal depolarization, confirming its functional role.

Conclusions:

TRPA1 is functionally expressed in L5 pyramidal neurons of the mammalian cortex.
Optovin provides a precise tool for investigating TRPA1 function in vivo.
This study enhances understanding of TRPA1's role in cortical circuits.