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A Multi-Platform Optical Sensor for In Vivo and In Vitro Algae Classification.

Chee-Loon Ng1, Qing-Qing Chen2, Jia-Jing Chua3

  • 1Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, CREATE Tower, #10-01, Singapore 138602, Singapore. kelvinng@smart.mit.edu.

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Summary

A new LED-induced fluorescence (LEDIF) instrument accurately identifies and quantifies major algal groups in water. This technology aids in ecological assessments and managing harmful algal blooms for better water resource management.

Keywords:
absorbancealgae sensorfluorescencein situ real-time algae classificationin vivo algae measurementoptical sensor

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Marine Biology

Background:

  • Accurate differentiation of algal groups is crucial for water body ecological and biogeochemical characterization.
  • Distinguishing beneficial algae from harmful algal bloom (HAB) species is vital for water resource management.

Purpose of the Study:

  • To introduce and validate a novel LED-induced fluorescence (LEDIF) instrument for algal group identification and quantification.
  • To assess the LEDIF instrument's performance in both laboratory and field conditions for various algal species.

Main Methods:

  • The LEDIF instrument was used for fluorescence, absorbance, and scattering measurements.
  • Extracted pigments from algal groups were analyzed, and cultivated algae were quantified using hemacytometry/cellometry and dry weight measurements.
  • In vivo measurements of different algal genera, concentrations, and mixed groups in humic acid were conducted, followed by field sample validation.

Main Results:

  • The LEDIF instrument demonstrated capability in measuring extracted biological pigments relevant to different algal groups.
  • Correlations between cell counts and dry weight were established for various algae.
  • In vivo measurements successfully classified and quantified different live algal groups, even in the presence of humic acid.

Conclusions:

  • The LEDIF instrument is a reliable tool for in vivo and in vitro identification and quantification of major algal groups.
  • This technology supports ecological monitoring, water quality assessment, and the management of harmful algal blooms.