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Updated: Mar 3, 2026

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
Published on: February 18, 2022
Leveraging microbial biosynthetic pathways for the generation of 'drop-in' biofuels
Amin Zargar1, Constance B Bailey2, Robert W Haushalter2
1Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, United States; QB3 Institute, University of California-Berkeley, 5885 Hollis Street, 4th Floor, Emeryville, CA 94608, United States.
Abstract:
Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and jet fuel (8%), 'drop-in' biofuels that replace these petrochemical sources are particularly attractive. In this review, we discuss the application of aldehyde decarbonylases to produce gasoline substitutes from fatty acid products, a recently crystallized reductase that could hydrogenate jet fuel precursors from terpene synthases, and the exquisite control of polyketide synthases to produce biofuels with desired physical properties (e.g., lower freezing points). With our increased understanding of biosynthetic logic of metabolic pathways, we discuss the unique advantages of fatty acid, terpene, and polyketide synthases for the production of bio-based gasoline, diesel and jet fuel.
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