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Antithrombin Activity of Erythrocyte Microvesicles
1Department of Gravitational Surgery and Hemodialysis, Volga Federal Medical Research Center, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia. levin@unn.ac.ru.
Bulletin of Experimental Biology and Medicine
|April 22, 2017
Summary
Erythrocyte-derived microvesicles show significant antithrombin activity, increasing during storage. These microvesicles inhibit blood clot formation and reduce optical density in assays.
Area of Science:
- Hematology
- Biochemistry
- Blood Coagulation
Background:
- Erythrocytes (red blood cells) are crucial for oxygen transport.
- Erythrocyte storage can lead to changes in their properties.
- Microvesicles are small particles released from cells.
Purpose of the Study:
- To investigate the antithrombin activity of erythrocytes and their derived microvesicles.
- To determine how erythrocyte storage affects this activity.
Main Methods:
- Coagulation assays were used to measure clot formation.
- Optical methods utilizing chromogenic substrates assessed enzyme activity.
- Erythrocytes and microvesicles were isolated at various storage time points (7, 14, 21, 28 days).
Main Results:
- Erythrocyte-derived microvesicles significantly decelerated fibrin clot formation.
- This inhibition occurred with and without heparin, indicating direct antithrombin effects.
- Microvesicles reduced the optical density of chromogenic substrates, confirming enzyme inhibition.
- Antithrombin activity of microvesicles increased progressively during erythrocyte storage.
Conclusions:
- Erythrocyte-derived microvesicles possess notable antithrombin properties.
- The antithrombin activity of these microvesicles is enhanced by prolonged erythrocyte storage.
- These findings have implications for understanding blood storage and coagulation dynamics.
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