Related Experiment Video
Updated: Mar 3, 2026

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
Noumeavirus replication relies on a transient remote control of the host nucleus
Elisabeth Fabre1, Sandra Jeudy1, Sébastien Santini1
1Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique &Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479), 13288 Marseille Cedex 9, France.
Abstract:
Acanthamoeba are infected by a remarkable diversity of large dsDNA viruses, the infectious cycles of which have been characterized using genomics, transcriptomics and electron microscopy. Given their gene content and the persistence of the host nucleus throughout their infectious cycle, the Marseilleviridae were initially assumed to fully replicate in the cytoplasm. Unexpectedly, we find that their virions do not incorporate the virus-encoded transcription machinery, making their replication nucleus-dependent. However, instead of delivering their DNA to the nucleus, the Marseilleviridae initiate their replication by transiently recruiting the nuclear transcription machinery to their cytoplasmic viral factory. The nucleus recovers its integrity after becoming leaky at an early stage. This work highlights the importance of virion proteomic analyses to complement genome sequencing in the elucidation of the replication scheme and evolution of large dsDNA viruses.
Related Concept Videos
Retrovirus Life Cycles
Viruses with RNA Genomes
Size and Structure of Viral Genomes
What are Viruses?
Retroviruses
Viral Structure

