Related Experiment Video
Updated: Mar 3, 2026

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
Published on: November 23, 2019
Pyrophosphate as substrate for alkaline phosphatase activity: A convenient flow-injection chemiluminescence assay
Qingfeng Zhang1,2, Cuiyun Zhang1,2,3, Meiding Yang1,2
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, People's Republic of China.
Abstract:
A sensitive and convenient flow-injection chemiluminescence (FI-CL) turn-on assay for alkaline phosphatase (ALP) activity without any label and synthesis is developed. Cu2+ can catalyze the luminol-H2 O2 CL reaction. Pyrophosphate (PPi) can chelate Cu2+ and therefore the Cu2+ -mediated luminol-H2 O2 CL reaction is inhibited. The addition of ALP can catalyze the hydrolysis of PPi into phosphate ions, Cu2+ is released and the chemiluminescence recovers. A detection limit of 1 mU/mL ALP is obtained.

