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Related Experiment Videos

Antidiuretic hormone moves membranes.

J S Handler1

  • 1Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.

The American Journal of Physiology
|September 1, 1988
PubMed
Summary
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Antidiuretic hormone (ADH) regulates water permeability in kidney collecting ducts by altering apical membrane particle aggregates. ADH withdrawal triggers endocytosis, retrieving these water-permeable membrane structures.

Area of Science:

  • Cell biology
  • Renal physiology
  • Membrane biophysics

Background:

  • The apical plasma membrane of the urinary collecting duct is crucial for regulating water reabsorption.
  • Antidiuretic hormone (ADH) significantly modulates water permeability in response to hydration status.

Purpose of the Study:

  • To review the molecular events at the apical membrane of toad urinary bladder and mammalian collecting duct during changes in water permeability induced by ADH.
  • To elucidate the structural and protein changes associated with ADH action and withdrawal.

Main Methods:

  • Freeze-fracture electron microscopy to visualize membrane structure.
  • Covalent labeling of apical membrane proteins.
  • Endocytosis studies during ADH withdrawal.

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Main Results:

  • ADH increases apical membrane particle aggregates and fused vesicles (aggrephores) in toad bladder.
  • ADH withdrawal leads to endocytosis of particle aggregate-containing membrane.
  • Specific proteins appear in the apical membrane during ADH response and are retrieved upon withdrawal.

Conclusions:

  • The apical membrane undergoes significant structural and protein composition changes in response to ADH.
  • Endocytosis is the mechanism for retrieving water-permeable membrane upon ADH withdrawal.
  • The review considers controversial aspects of ADH-mediated water transport.