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An improved procedure for the rapid one-step-cloning of full-length viroid cDNA
1Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried bei München, Federal Republic of Germany.
Archives of Virology
|January 1, 1988
Abstract:
The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multi-ion transformation is applied.