Analysis of PM_2.5-induced cytotoxicity in human HaCaT cells based on a microfluidic system

Human exposure to PM_2.5 causes several adverse health effects. Skin is the first barrier against harmful environmental substances and can directly contact with PM_2.5, but there is no study about PM_2.5-induced cytotoxicity in human skin cells on the molecular level partially due to the shortcomings of traditional research methods. In present study, we established a microfluidic system including a cell culture chip integrated with a high-throughput protein microarray chip to investigate the mechanism of PM_2.5-mediated cytotoxicity in human HaCaT cells. We found that PM_2.5 was lodged inside the cytoplasm, mitochondria and nucleus of HaCaT cells by TEM. Flow cytometry analysis indicated that the cell apoptosis rate increased from 0.49% to 53.4%. The results of protein microarray showed that NF-κB and NALP3 signal transductions were activated in HaCaT cells after PM_2.5 stimulations, up-regulating the expression of IL-1β and IL-6, which resulted in inflammatory response in HaCaT cells. Our findings provide a molecular insight into PM_2.5-induced skin injury.