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Effect of high temperature on Aedes albopictus cells infected with Mayaro virus
M G Carvalho1, M A Rebello, J M Mezencio
1Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Abstract:
The multiplication of Mayaro virus in Aedes albopictus cells was drastically inhibited after incubation at 37 degrees C. The effect of short-term exposure of infected cells to high temperatures (heat shock) produced a preferential translation of the heat shock messengers when compared to the viral mRNAs. When cells were shifted back to 28 degrees C (the optimum growth temperature for Aedes albopictus cells), preferential translation of viral mRNA occurred. Although the infected cells were programmed for preferential translation of viral messengers, the thermal treatment was able to shift the translational machinery towards synthesis of heat shock proteins.
Insights
High temperatures inhibit Mayaro virus replication in Aedes albopictus cells by altering protein synthesis. Heat shock shifts cellular machinery from viral messenger RNA translation to heat shock protein production.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Mayaro virus (MAYV) is an arbovirus transmitted by Aedes mosquitoes.
- Mosquito cell lines are crucial for studying arbovirus replication and pathogenesis.
- Temperature stress can significantly impact viral replication and host-cell interactions.
Purpose of the Study:
- To investigate the effect of high temperatures on Mayaro virus multiplication in Aedes albopictus cells.
- To examine the impact of heat shock on viral and cellular messenger RNA translation.
- To understand the host-pathogen response to thermal stress at the translational level.
Main Methods:
- Infection of Aedes albopictus cells with Mayaro virus.
- Incubation of infected cells at 37°C (heat shock) and 28°C (optimal growth temperature).
- Analysis of messenger RNA translation patterns under different temperature conditions.
Main Results:
- Mayaro virus multiplication was significantly inhibited at 37°C.
- Short-term heat shock at 37°C led to preferential translation of heat shock messengers over viral mRNAs.
- Upon return to 28°C, preferential translation of viral mRNA resumed, despite prior heat shock treatment.
Conclusions:
- Thermal stress, specifically heat shock, can disrupt Mayaro virus replication in Aedes albopictus cells.
- Heat shock alters the cellular translational machinery, favoring the synthesis of heat shock proteins over viral proteins.
- These findings highlight a potential mechanism by which temperature fluctuations can influence arbovirus transmission dynamics.