Related Experiment Video
Updated: Jul 16, 2026

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
Published on: March 31, 2022
Cooperativity and hierarchical levels of functional organization in the SV40 enhancer
C Fromental1, M Kanno, H Nomiyama
1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France.
Abstract:
We have investigated the cell-specific activity of the GT-IIC, GT-I, Sph-II, Sph-I, and octamer motifs of the SV40 enhancer in four cell lines (HeLa cells, MPC11 plasmocytoma B cells, and non-differentiated and retinoic acid-differentiated F9 embryonal carcinoma cells). Our present results reveal the existence of three classes of motifs that interact with cell-specific enhancer factors but that have no enhancer activity of their own: those (class A) that generate enhancer activity following oligomerization of tandem repeats of the motif; those (class B) that cannot enhance transcription when oligomerized as a tandem repeat but whose association with a second motif results in transcription activation after oligomerization; and those (class C) that exhibit enhancer activity when a single copy of the motif is oligomerized. Three levels of functional organization of enhancers can also be defined. The possible implications for enhancer functions are discussed.
Related Concept Videos
RNA Polymerase II Accessory Proteins
Cooperative Binding of Transcription Regulators
Co-activators and Co-repressors
RNA Polymerase II Accessory Proteins
Cooperative Binding of Transcription Regulators
Co-activators and Co-repressors

