Enzymatic synthesis of isomaltotriose palmitate and evaluation of its emulsifying property

Enzymatic syntheses of oligosaccharide fatty acid esters are important owing to their wide range of industrial applications in the food, cosmetic, and pharmaceutical industries. Transesterification of isomaltotriose and palmitic acid vinyl ester, catalyzed by the metalloprotease thermolysin, was performed in organic solvents. The process parameters (reaction time and temperature) were optimized to achieve the highest yield of isomaltotriose palmitate (IP). The water content of the reaction system played a key role in the acylation of isomaltotriose. Dimethyl sulfoxide was thought to be the most suitable reaction medium by taking the degree of substitution of the modified isomaltotriose into account. The optimum reaction time, temperature, water content, and enzyme concentration were 24h, 45°C, 40%, and 0.05%, respectively, under which the product yield was as high as 89.7%. The enzyme operational stability study showed that thermolysin retained 51.5% of its initial activity for the synthesis of IP (even after repeated use for 72h). Moreover, test results showed that the emulsifying capacity and emulsion stability of IP are 107.5mL oil/g ester and 16.3%, respectively.