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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Optimizing Sample Preparation for Cryogenic Electron Microscopy
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A novel storage system for cryoEM samples.

Giovanna Scapin1, Winifred W Prosise1, Michael K Wismer2

  • 1Department of Structural Chemistry and Chemical Biotechnology, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ 07033, United States.

Journal of Structural Biology
|April 24, 2017
PubMed
Summary

A new CryoEM grid box storage system simplifies sample management using crystal pucks. This method enhances tracking and retrieval, benefiting cryo-electron microscopy labs, especially large facilities.

Keywords:
AutomationCryoTEMGrid storagePucks

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Area of Science:

  • Structural Biology
  • Biophysics
  • Cryo-Electron Microscopy

Background:

  • Cryo-electron microscopy (CryoEM) requires robust sample handling and storage solutions.
  • Efficient management of numerous samples is crucial for large-scale structural biology projects.
  • Current methods for cryoEM grid storage can be cumbersome for tracking and retrieval.

Purpose of the Study:

  • To introduce a novel storage system for CryoEM grid boxes.
  • To enhance the ease of sample labeling, tracking, and retrieval in CryoEM workflows.
  • To provide a scalable solution suitable for diverse CryoEM laboratory settings.

Main Methods:

  • Adaptation of the crystal puck system, commonly used in X-ray crystallography.
  • Implementation of standardized labeling and tracking protocols for grid boxes within the puck system.
  • Design focused on compatibility with existing CryoEM laboratory infrastructure.

Main Results:

  • Demonstrated simplification of the sample labeling process.
  • Improved efficiency in tracking and retrieving specific CryoEM samples.
  • Successful integration of the system into a CryoEM laboratory setting.

Conclusions:

  • The proposed CryoEM grid box storage system offers a practical and efficient solution for sample management.
  • The system's foundation on established crystallographic pucks ensures familiarity and ease of adoption.
  • This innovation is particularly advantageous for large CryoEM facilities managing high sample volumes from multiple sources.