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Related Experiment Videos

HLA-DR gene expression in a proliferating human thyroid cell clone (12S).

R D Cone1, M Platzer, L A Piccinini

  • 1Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.

Endocrinology
|October 1, 1988
PubMed
Summary

Researchers immortalized human thyroid cells using a retroviral vector. These cells respond to thyroid-stimulating hormone (TSH) and gamma-interferon, offering a model for studying thyroid function and immune responses.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Immunology

Background:

  • Establishing immortalized human thyroid cell lines is crucial for studying thyroid physiology and disease.
  • Understanding the regulation of human leukocyte antigen (HLA) class II expression in thyroid cells is important for autoimmune thyroid diseases.

Purpose of the Study:

  • To create an immortalized human thyroid cell line responsive to TSH and gamma-interferon.
  • To investigate the potential of these cells for studying TSH receptor immunity and HLA gene regulation.

Main Methods:

  • Retroviral vector transduction with adenovirus E1A oncogene and neomycin phosphotransferase gene.
  • Culture and selection of infected human fetal thyroid cells in a hormone-supplemented medium with G418 antibiotic.
  • Assessment of TSH-mediated cAMP generation and gamma-interferon-induced HLA class II antigen expression via mRNA analysis and flow cytometry.

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Main Results:

  • An immortalized human thyroid cell clone (12S) was established, exhibiting TSH-mediated cAMP generation.
  • Clone 12S showed sensitivity to low concentrations of bovine TSH (bTSH) but no TSH influence on DNA synthesis.
  • Recombinant human gamma-interferon induced HLA DR alpha-chain mRNA and surface HLA-DR antigen expression in clone 12S.

Conclusions:

  • The immortalized 12S cell line serves as a valuable tool for studying TSH receptor immune responses.
  • This cell line facilitates long-term investigations into the regulation of human thyroid HLA gene expression.