Transforming growth factor-beta 1 signaling regulates neuroinflammation and apoptosis in mild traumatic brain injury

Mild traumatic brain injury (mTBI) is a low-level injury, which often remains undiagnosed, and in most cases it leads to death and disability as it advances as secondary injury. Therefore, it is important to study the underlying signaling mechanisms of mTBI-associated neurological ailments. While transforming growth factor-beta1 (TGF-β1) has a significant role in inflammation and apoptosis in myriads of other pathophysiological conditions, the precise function of increased TGF-β1 after mTBI is unknown. In this study, our objective is to study the physiological relevance and associated mechanisms of TGF-β1-mediated inflammation and apoptosis in mTBI. Using an in vitro stretch-injury model in rat neuronal cultures and the in vivo fluid percussion injury (FPI) model in rats, we explored the significance of TGF-β1 activation in mTBI. Our study demonstrated that the activation of TGF-β1 in mTBI correlated with the induction of free radical generating enzyme NADPH oxidase 1 (NOX1). Further, using TGF-β type I receptor (TGF-βRI) inhibitor SB431542 and transfection of TGF-β1 siRNA and TGF-β antagonist Smad7, we established the neuroinflammatory and apoptotic role of TGF-β1 in mTBI. Inhibition of TGF-βRI or TGF-β1 diminished TGF-β1-induced inflammation and apoptosis. Further, the enhanced TGF-β1 activation increased the phosphorylation of R-Smads including Smad2 and Smad3 proteins. By immunofluorescence, western blotting, ELISA and TUNEL experiments, we demonstrated the up-regulation of pro-inflammatory cytokines IL-1β and TNF-α and apoptotic cell death in neurons. In conclusion, this study could establish the significance of TGF-β1 in transforming the pathophysiology of mTBI into secondary injury.