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Correction: Onoiu et al. Circulating Lipid Profiles Indicate Incomplete Metabolic Recovery After Weight Loss, Suggesting the Need for Additional Interventions in Severe Obesity. <i>Biomolecules</i> 2025, <i>15</i>, 1112.

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Brown Adipose Tissue Bioenergetics: A New Methodological Approach.

María Calderon-Dominguez1,2, Martín Alcalá3, David Sebastián4,5

  • 1Department of Biochemistry and Physiology Institut de Biomedicina de la Universitat de Barcelona (IBUB) Universitat de Barcelona E-08028 Barcelona Spain.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|April 25, 2017
PubMed
Summary

Researchers developed a new method to measure energy production in brown adipose tissue (BAT) directly within the tissue. This approach offers a realistic view of thermogenesis and metabolic function for obesity and diabetes research.

Keywords:
bioenergeticsbrown adipose tissuemitochondriaoxygen consumption rateseahorse

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Area of Science:

  • Metabolic Physiology
  • Adipose Tissue Biology
  • Mitochondrial Bioenergetics

Background:

  • Brown adipose tissue (BAT) plays a crucial role in energy expenditure and thermogenesis.
  • Its therapeutic potential for metabolic diseases like obesity and diabetes is significant.
  • Current methods for measuring BAT bioenergetics lack tissue context.

Purpose of the Study:

  • To develop and optimize a method for quantifying mitochondrial bioenergetics directly within intact brown adipose tissue.
  • To establish a realistic assessment of BAT's physiological respiration and energy metabolism.
  • To enable the study of factors influencing thermogenesis and fuel utilization in BAT.

Main Methods:

  • Utilized XF Seahorse Technology for direct measurement of bioenergetics in BAT explants.
  • Optimized key parameters including explant weight, inhibitor concentration, and incubation time.
  • Compared oxygen consumption rates between BAT and white adipose tissue (WAT).

Main Results:

  • Established a reliable method for direct bioenergetics measurement in BAT.
  • Identified proton leak as the primary contributor to basal oxygen consumption in BAT.
  • Demonstrated higher basal oxygen consumption in BAT compared to WAT, with a positive response to beta-adrenergic stimulation.

Conclusions:

  • Directly measuring bioenergetics in whole BAT provides a physiologically relevant understanding of its function.
  • This method facilitates the investigation of novel thermogenesis activators and substrate utilization.
  • The technique holds promise for advancing research into obesity and diabetes treatments.