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Multiple base-pair mutations in yeast.

D M Hampsey1, J F Ernst, J W Stewart

  • 1Department of Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642.

Journal of Molecular Biology
|June 5, 1988
PubMed
Summary
This summary is machine-generated.

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Yeast CYC1 gene mutations were analyzed, revealing that while most are single base changes, about 10% involve multiple mutations occurring in clusters. These clustered mutations suggest concerted mutational events rather than independent ones.

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Research

Background:

  • The CYC1 locus in Saccharomyces cerevisiae encodes iso-1-cytochrome c.
  • Understanding mutation mechanisms is crucial for genetic studies.

Purpose of the Study:

  • To investigate nucleotide changes in forward and reverse mutations at the CYC1 locus.
  • To characterize the mechanisms underlying multiple base-pair mutations.

Main Methods:

  • Sequencing of the mutated iso-1-cytochrome c gene product.
  • Direct sequencing of relevant DNA segments from Saccharomyces cerevisiae.

Main Results:

  • The majority of mutations are single base-pair changes.
  • Approximately 10% of mutations involve multiple base-pair changes, often clustered within six nucleotides.

Related Experiment Videos

  • Multiple mutations were classified into five categories, including direct/inverted repeats, tandem substitutions, frameshifts, and recombination events.

    • Conclusions:

    • Multiple base-pair mutations in the CYC1 locus appear to arise from single, concerted events.
    • Diverse mechanisms contribute to multiple base-pair mutations, including repeat formation and recombination.
    • The study proposes specific mechanisms for observed mutation classes.