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Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis
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Tissue factor-dependent coagulation activation by heme: A thromboelastometry study.
Gleice Regina de Souza1, Bidossessi Wilfried Hounkpe1, Maiara Marx Luz Fiusa1
1Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
Plos One
|April 25, 2017
Summary
Heme activates blood coagulation ex vivo, impacting early clot formation. This effect is partly dependent on tissue factor, potentially explaining hemostatic imbalances in conditions like sickle cell disease.
Area of Science:
- Biochemistry
- Hematology
- Physiology
Background:
- Heme is a known inflammation trigger.
- Heme's role in hemostasis is complex, with both pro- and anti-coagulant effects observed.
- The net impact of heme on hemostatic balance and its clinical relevance require further investigation.
Purpose of the Study:
- To investigate the effect of heme on hemostasis using a global assay.
- To determine the dependence of heme-induced coagulation on tissue factor.
Main Methods:
- Citrated whole blood from healthy donors was stimulated with heme or vehicle.
- Rotational thromboelastometry was performed to assess global hemostasis.
- Inhibitory antibodies against tissue factor were used to evaluate its role.
Main Results:
- Heme induced ex vivo coagulation activation in whole blood.
- Heme primarily affected parameters related to the initial stages of clot formation.
- Heme-induced activation was partially dependent on hematopoietic tissue factor.
Conclusions:
- Heme activates coagulation in whole blood in a tissue factor-dependent manner.
- These findings may elucidate hemostatic disturbances in heme-releasing conditions, such as sickle cell disease.
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