Ion concentration effect (Na⁺ and Cl^-) on lipid vesicle formation

Lipid vesicle formation is known to be suppressed in salt solutions, but the mechanism of this phenomenon remains unclear. In order to better understand this issue, the effect of salt concentrations (0-800mM) of sodium chloride on the behavior of L-α-phosphatidylcholine (PC) in aqueous solution was investigated in this work. The results showed that fusion among vesicles, micelles and bilayers may be essential for vesicle formation. With addition of ions and an increase in ion concentration, the lipids became constrained in lateral movement and packed increasingly tightly. The resulted hard supported phospholipid bilayers (SPBs) were thus more difficult to detach from the substrate to form vesicles. These phenomena were tried to be explained at molecular level. Hydrophobic effect is the original cause of lipid vesicle formation, which in fact is absence of attraction between the involved substances. That is to say, the stronger the 3D network was bounded in the medium, the stronger the hydrophobic repulsion on the lipids would be. This might be one reason for the suppression of vesicle formation in salt solution.