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Updated: Mar 3, 2026
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment
Tran Thi Ngoc Dung1, Pham Thanh Duy1, October M Sessions2
1The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University, Clinical Research Unit, 764 Vo Van Kiet, Quan 5, Ho Chi Minh City, Vietnam.
Background:
Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material.
Methods:
To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents.
Results:
Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30).
Conclusions:
This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems.
Trial Registration (Prospectively Registered):
Current Controlled Trials ISRCTN88101063 . Date of registration: 14/06/2012.
