Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversible control of CAR T cells through PROTAC compound targeting bromodomain mutant.

Molecular therapy. Oncology·2026
Same author

SSEL-CPP: A SHAP-based feature-selection ensemble learning framework identifies molecular properties of cell-penetrating peptides.

Protein science : a publication of the Protein Society·2026
Same author

Nano formulation of Sophora japonica extract stimulates osteogenic activity in osteoblasts.

Journal of food science and technology·2026
Same author

CONTRA-IL6: an interpretable hybrid convolutional neural network and Transformer framework for accurate prediction of interleukin-6-inducing peptides using protein language models.

Briefings in bioinformatics·2026
Same author

Integrative Peptide Drug Development: Chemical Engineering, AI-Driven Design, and Cell-Penetrating Peptides.

Pharmaceutics·2026
Same author

Corrigendum to Angiopoietin-2 exacerbates cardiac hypoxia and inflammation after myocardial infarction.

The Journal of clinical investigation·2026

Related Experiment Video

Updated: Mar 3, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
05:57

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

4.0K

Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and

Tae Hwan Shin1,2, Seungah Lee3, Ki Ryung Choi2,4

  • 1Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea.

Scientific Reports
|April 26, 2017
PubMed
Summary

Quality of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) declines post-dissociation. Reduced deformability, increased reactive oxygen species (ROS), and altered amino acid profiles indicate loss of freshness, emphasizing the need for freshly prepared cells in therapies.

More Related Videos

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

5.4K
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
08:58

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

9.7K

Related Experiment Videos

Last Updated: Mar 3, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
05:57

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

4.0K
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

5.4K
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
08:58

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

9.7K

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Cellular Physiology

Background:

  • Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) show therapeutic promise.
  • Assessing hBM-MSC quality before transplantation is crucial but challenging.

Purpose of the Study:

  • To investigate changes in hBM-MSC quality over time after dissociation.
  • To evaluate parameters including deformability, gene expression, amino acid profiles, and membrane fluidity.

Main Methods:

  • Measurement of cell deformability and size.
  • Analysis of gene expression related to reactive oxygen species (ROS) generation and amino acid metabolism.
  • Assessment of intracellular ROS levels and membrane fluidity using Laurdan generalized polarization.
  • Evaluation of N-acetyl-L-cysteine treatment effects.

Main Results:

  • Decreased cell deformability and size observed after 6 and 12 hours of storage.
  • Increased intracellular ROS levels correlated with altered gene expression.
  • Changes in amino acid profiles and membrane fluidity were noted over time.
  • N-acetyl-L-cysteine treatment reversed some membrane fluidity changes.

Conclusions:

  • hBM-MSC quality and freshness diminish significantly after dissociation from culture.
  • Time-dependent alterations in cellular properties impact cell viability and function.
  • Clinical applications should prioritize the use of freshly prepared (trypsinized) hBM-MSCs.