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Published on: January 20, 2023
Development and characterization of EST-SSR markers in Stipa breviflora (Poaceae)
Jing Ren1, Zhi-Zhen Su1, Zhen-Hua Dang1
1School of Ecology and Environmental Sciences, Inner Mongolia University, Hohhot, People's Republic of China.
Premise Of The Study:
Stipa breviflora (Poaceae) is one of the dominant species of the desert steppe in the eastern Eurasian grasslands. Simple sequence repeat (SSR) markers were developed for use in genetic diversity studies of this species.
Methods And Results:
A total of 1954 potentially polymorphic loci were obtained by comparing transcriptome data of eight different S. breviflora individuals. We selected 81 loci to verify polymorphism and 63 loci amplified, of which 21 loci exhibited polymorphism. The number of alleles per locus varied from two to 24, the observed heterozygosity ranged from 0.083 to 0.958, and the expected heterozygosity ranged from 0.396 to 0.738.
Conclusions:
These newly identified SSR loci can be used for population genetic and landscape genetic studies of S. breviflora. In addition, 14 loci also amplified in six related Stipa species (S. grandis, S. krylovii, S. bungeana, S. aliena, S. gobica, and S. purpurea).
