Amylase-assisted extraction and antioxidant activity of polysaccharides from Gracilaria lemaneiformis
Shengjun Wu1,2,3,4, Mingsheng Lu1,2,3,4, Shujun Wang5,6,7,8
1Marine Resources Development Institute of Jiangsu, Huaihai Institute of Technology, 59 Cangwu Road, Haizhou, Lianyungang, 222005, China.
Abstract:
Gracilaria lemaneiformis (G. lemaneiformis) is rich in polysaccharides that have many functional activities. In this study, the thermostable α-amylase-assisted extraction and antioxidant activity of water-soluble polysaccharides from G. lemaneiformis were investigated. The extraction conditions were optimized as follows: time, 40 min; temperature, 95 °C; pH, 5 and enzyme amount, 6000 U/g, under which the yield of G. lemaneiformis polysaccharides (GPs) reached 49.15% dry base. The GPs were characterized by monosaccharide composition, FTIR spectrum, UV spectrum and antioxidant activities. The results indicate that the GPs had strong hydroxyl radical activity at a concentration of 100 μg/mL and could be used as potential antioxidants.
