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Plant Tissues

Plant Tissues

Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...

Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

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Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.

Erin E Sparks¹, Philip N Benfey²

¹Department of Biology and Howard Hughes Medical Institute, Duke University, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 26, 2017

Summary

This study details methods for isolating plant root cells using fluorescence-activated cell sorting (FACS) and analyzing their gene expression through RNA sequencing for developmental insights.

Keywords:

Fluorescence-activated cell sorting Protoplast RNAseq Roots Transcriptomics

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Area of Science:

Plant biology
Molecular biology
Genomics

Background:

Understanding plant development and environmental responses requires spatiotemporal transcriptome data from specific tissues.
Fluorescence-activated cell sorting (FACS) is a valuable technique for isolating distinct cell populations.

Purpose of the Study:

To outline a detailed methodology for isolating plant root protoplasts using FACS.
To describe subsequent transcriptome profiling of these isolated cells via RNA sequencing.

Main Methods:

Isolation of root protoplasts from plants.
Application of fluorescence-activated cell sorting (FACS) for cell purification.
Transcriptome profiling using RNA sequencing (RNA-seq).

Main Results:

Successful isolation of viable root protoplasts using FACS.
Generation of high-quality RNA from sorted protoplasts.
Obtained transcriptome data provides insights into root cell-specific gene expression.

Conclusions:

FACS is an effective method for isolating specific plant root cell types for transcriptomic analysis.
This approach enables detailed study of gene expression patterns during plant development and environmental interactions.