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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Related Experiment Video

Updated: Mar 3, 2026

Author Spotlight: Multiplex Immunohistochemistry for Understanding Immune Regulation by Uterine NK Cells in Pregnancy
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Aberrantly expressed long noncoding RNAs in recurrent implantation failure: A microarray related study.

Li-Juan Fan1, Hong-Jing Han1, Jing Guan1

  • 1a Department of Reproductive Medical Center , Peking University People's Hospital , Beijing , China.

Systems Biology in Reproductive Medicine
|April 26, 2017
PubMed
Summary

Long noncoding RNAs (lncRNAs) show altered expression in women with recurrent implantation failure (RIF). These lncRNAs may play a role in regulating endometrial receptivity, impacting fertility outcomes.

Keywords:
Endometrial receptivitylong noncoding RNAmicroarrayrecurrent implantation failure

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Area of Science:

  • Reproductive biology and genetics
  • Noncoding RNA research
  • Genomics and transcriptomics

Background:

  • Long noncoding RNAs (lncRNAs) were historically considered transcriptional noise but are now recognized for their crucial roles in human biology.
  • Recurrent implantation failure (RIF) is a complex condition affecting fertility, with underlying molecular mechanisms still being elucidated.
  • Endometrial receptivity, the window of implantation (WOI), is critical for successful embryo implantation.

Purpose of the Study:

  • To profile the endometrial lncRNA expression patterns in women with RIF.
  • To identify dysregulated lncRNA transcripts in RIF endometrium.
  • To predict the functions of genes associated with dysregulated lncRNAs and their potential role in endometrial receptivity.

Main Methods:

  • Microarray analysis of endometrial lncRNA expression in RIF patients and controls.
  • Quantitative real-time PCR (RT-qPCR) to validate microarray findings.
  • lncRNA-mRNA co-expression analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and lncRNA-transcription factor (TF) analyses.

Main Results:

  • A total of 197 dysregulated lncRNA transcripts were identified in RIF endometrium compared to controls.
  • RT-qPCR validated the differential expression of eight selected lncRNA transcripts.
  • Coexpressed mRNA transcripts were associated with pathways potentially affecting endometrial receptivity, such as cell adhesion.
  • Potential TF targets for the dysregulated lncRNAs were predicted.

Conclusions:

  • Endometrial lncRNA expression profiles differ significantly between women with RIF and those with successful pregnancies.
  • lncRNAs are implicated as potential regulators of endometrial receptivity.
  • These findings suggest lncRNAs as potential diagnostic or therapeutic targets for RIF.