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Codon Usage Optimization and Construction of Plasmid Encoding Iranian Human Papillomavirus Type 16 E7 Oncogene for
Amir Hossein Mohseni1, Vadood Razavilar, Hossein Keyvani
1Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Iran. Email: v-razavilar@srbiau.ac.ir, ah.mohseni@srbiau.ac.ir.
Asian Pacific Journal of Cancer Prevention : APJCP
|April 27, 2017
Summary
This study identified novel HPV 16 E7 gene variations in Iranian women, optimizing the E7 sequence for potential vaccine development.
Area of Science:
- Virology
- Molecular Biology
- Vaccine Development
Background:
- Human Papillomavirus (HPV) 16 intratypic variations influence oncogenic potential and geographic distribution.
- Understanding HPV 16 E7 gene variations is crucial for developing effective vaccines.
Purpose of the Study:
- To investigate nucleotide modifications and optimize the HPV 16 E7 gene region in Iranian women.
- To construct an expression vector for the optimized HPV 16 E7 oncoprotein.
Main Methods:
- Analysis of cervical biopsies from HPV 16 positive cancer patients using PCR, cloning, and sequencing.
- Codon optimization of the E7 gene for Lactococcus lactis subsp. cremoris MG1363.
- Construction of the pNZ8148 expression vector encoding the optimized E7 oncoprotein.
Main Results:
- The most frequent variant observed was C196T, resulting in an R66W amino acid change.
- A common variant T234G was found, but without amino acid alteration.
- Codon optimization significantly reduced G+C content from 43.1% to 34.0% and improved codon adaptation index (CAI).
Conclusions:
- This study provides the first report of HPV 16 E7 genetic variations in Iran.
- The constructed pNZ8148-HPV 16-opti E7 plasmid shows potential as a future vaccine candidate.
