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Diversity and evolutionary aspects of mucin binding (MucBP) domain repeats among Lactobacillus plantarum group

Sundru Manjulata Devi1, Prakash M Halami1

  • 1Microbiology and Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysuru 570020, India.

Systematic and Applied Microbiology
|April 27, 2017
PubMed

Insights

Mucin binding protein (Mub) diversity in Lactobacillus plantarum-group (LPG) strains influences gene expression and adherence. This study characterized mub gene variations, revealing insights into LPG strain selection for enhanced gut health.

Area of Science:

  • Microbiology
  • Genetics

Background:

  • Mucin binding protein (Mub) in Lactobacillus plantarum-group (LPG) is a key adherence factor and potential marker gene.
  • Strain-specific diversity in the mub gene, including variations in MucBP domain repeats, has been observed in LPG.

Purpose of the Study:

  • To investigate the genetic diversity of the mub gene in LPG strains.
  • To correlate mub gene structure with its expression levels and adherence properties.
  • To understand the evolutionary origins of specific mub gene domains.

Main Methods:

  • Analysis of mub gene sequences from eight LPG strains to identify variations in MucBP domain repeats.
  • Real-time PCR to quantify mub gene expression.
  • Detection of integrase and transposase genes in flanking regions.
  • Phylogenetic analysis of mub gene domains.

Main Results:

  • LPG strains were classified into Type I, II, and III based on mub gene domain repeat variations.
  • Mub gene expression increased with the number of MucBP repeats, with a 67.64-fold change observed in a strain with four repeats.
  • Integrase and transposase genes flanking the mub gene contributed to observed strain diversity.
  • Phylogenetic analysis indicated that specific domains evolved from related Lactobacillus species.

Conclusions:

  • Genetic diversity in the mub gene, particularly domain repeats, significantly impacts LPG gene expression and adherence.
  • The study identified an efficient LPG strain with high adherence potential.
  • Understanding mub gene variations provides insights into LPG evolution and strain selection for probiotic applications.
Keywords:
AdhesionIntegraseLactobacillus paraplantarumMucBP domain repeatProbioticTransposase

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