Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T cell-associated immunity induced by heterologous recombinant BCG and purified protein vaccination confers cross-variant protection against SARS-CoV-2.

Human vaccines & immunotherapeutics·2026
Same author

Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation.

The Journal of membrane biology·2026
Same author

Cellular and molecular responses of <i>Cryptococcus</i> to brilacidin.

Microbiology spectrum·2026
Same author

Single-cell proteomics: When individual cells tell population stories.

Trends in biochemical sciences·2026
Same author

Quinoa bioester application shifts human skin proteome toward molecular profiles associated with younger age.

Communications biology·2026
Same author

Canonical WNT signalling governs Echinococcus metacestode development.

PLoS pathogens·2026

Related Experiment Video

Updated: Mar 3, 2026

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus
09:04

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus

Published on: March 28, 2025

811

Comparative proteomics of hydatid fluids from two Echinococcus multilocularis isolates.

Karina M Monteiro1, Karina R Lorenzatto2, Jeferson C de Lima2

  • 1Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Departamento de Biologia Molecular e Celular, Instituto de Biociências, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.

Journal of Proteomics
|April 27, 2017
PubMed
Summary

This study compared hydatid fluid proteins from two Echinococcus multilocularis isolates, revealing differences in parasite and host proteins. These variations may indicate distinct biological features and offer targets for diagnosing and treating alveolar echinococcosis.

Keywords:
Alveolar echinococcosisExcretion/secretion productsHydatid fluidParasite proteomicsParasite-host interactions

More Related Videos

Transient Transduction of the Strobilated Forms of Echinococcus granulosus
13:25

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Published on: September 16, 2022

3.6K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.7K

Related Experiment Videos

Last Updated: Mar 3, 2026

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus
09:04

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus

Published on: March 28, 2025

811
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
13:25

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Published on: September 16, 2022

3.6K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.7K

Area of Science:

  • Parasitology
  • Proteomics
  • Molecular Biology

Background:

  • Hydatid fluid (HF) from Echinococcus multilocularis contains parasite and host proteins crucial for infection.
  • HF is a key source for echinococcosis immunodiagnosis.
  • Intraspecific variation in E. multilocularis isolates influences infection dynamics.

Purpose of the Study:

  • To compare the proteomic composition of HF from two E. multilocularis isolates (EmH95 and EmG8065).
  • To identify qualitative and quantitative protein differences between isolates.
  • To correlate protein profiles with biological features like growth and fertility.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for proteomic analysis.
  • HF samples from E. multilocularis isolates H95 and G8065 were analyzed.
  • Protein abundance and exclusivity were assessed for parasite and medium-derived proteins.

Main Results:

  • 446 unique proteins were identified in HF, with 88% of parasite origin.
  • Significant quantitative and qualitative differences were found in parasite proteins (antigens, enzymes, chaperones) between isolates.
  • Differential abundance of exogenous (medium) proteins was also observed between isolates.

Conclusions:

  • Proteomic differences in HF reflect intraspecific variation in E. multilocularis isolates.
  • Identified proteins may serve as molecular markers for parasite growth and fertility.
  • These findings suggest potential targets for novel diagnostic and therapeutic strategies against alveolar echinococcosis.