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MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway

Wu M Wang1, Gang Lu1, Xian W Su1

  • 1Division of Neurosurgery, Department of Surgery, Prince of Wales HospitalThe Chinese University of Hong Kong, Hong Kong, China.

Frontiers in Cellular Neuroscience
|April 27, 2017
PubMed

Insights

MicroRNA-182 (miR-182) is crucial for neuronal development, promoting axon outgrowth and dendrite formation. It activates the AKT pathway, highlighting its role in neurodevelopment and potential in related disorders.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • MicroRNAs regulate complex mechanisms in neuronal development and maturation.
  • Neuronal maturation, including axon outgrowth and dendrite formation, is vital for brain function and linked to neurodevelopmental disorders.

Purpose of the Study:

  • To investigate the role of neuron-enriched microRNA-182 (miR-182) in regulating neuronal axon outgrowth and dendrite tree formation.
  • To elucidate the molecular pathways, including the PTEN/AKT pathway, involved in miR-182-mediated neuronal maturation.

Main Methods:

  • Overexpression and reduction of miR-182 in neuronal cells.
  • Analysis of neurofilament-M and neurofilament-L expression.
  • Assessment of AKT and PTEN phosphorylation.
  • Inhibition of AKT activity using LY294002.
  • Investigation of BCAT2 as a potential miR-182 target.

Main Results:

  • Overexpression of miR-182 promoted axon outgrowth and dendrite complexity, increasing neurofilament expression.
  • Reduced miR-182 inhibited neurite outgrowth.
  • miR-182 activated the AKT pathway by increasing AKT phosphorylation and inhibiting PTEN activity.
  • Inhibition of AKT suppressed axon outgrowth.
  • BCAT2 was identified as a potential target of miR-182, and its blockage promoted axon outgrowth and AKT activity.

Conclusions:

  • miR-182 plays a significant role in regulating neuronal axon outgrowth and dendrite maturation.
  • The PTEN/AKT pathway is involved in miR-182's regulation of neuronal development.
  • miR-182 represents a potential therapeutic target for neurodevelopmental disorders.
Keywords:
AKTBCAT2axon outgrowthdendritemicroRNA-182

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