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Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function

Jocelyn M Choo¹, Paul J Trim², Lex E X Leong¹

¹Infection and Immunity Theme, South Australian Health and Medical Research Institute, AdelaideSA, Australia.

Frontiers in Microbiology

|April 27, 2017

Summary

The mouse intestinal microbiome changes significantly across generations in a controlled facility, impacting experimental results. Understanding these microbiome dynamics is crucial for reproducible research.

Keywords:

C57BL/6J inbred mice fecal metabolome fecal microbiota mice generations microbiome variation

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Area of Science:

Microbiology
Genomics
Metabolomics

Background:

Inbred mice are vital models for human physiology research.
Murine intestinal microbiota significantly impacts experimental outcomes.
Microbiome dynamics in experimental mouse populations are poorly understood.

Purpose of the Study:

Investigate changes in the intestinal microbiome across six generations of C57BL/6J mice.
Assess the impact of a controlled facility environment on microbiome composition and function.
Relate microbiome shifts to experimental reproducibility.

Main Methods:

16S rRNA gene amplicon sequencing for fecal microbiota composition.
Liquid chromatography-mass spectrometry for fecal metabolome analysis.
Longitudinal assessment across multiple mouse generations.

Main Results:

Significant divergence in intestinal microbiota observed between founder and second-generation mice.
Inter-generational variance in bacterial taxa (Akkermansia, Turicibacter, Bifidobacterium) and fecal metabolome.
Microbiome and metabolome shifts correlated with facility acclimation and housing conditions.

Conclusions:

Mouse intestinal microbiome composition and function change across generations.
These microbiome dynamics introduce variance impacting experimental reproducibility.
Consideration of microbiome changes is essential for robust experimental design and interpretation.