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Related Experiment Video

Updated: Mar 3, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

Viral Vectors for Plant Genome Engineering.

Syed Shan-E-Ali Zaidi¹, Shahid Mansoor¹

¹Molecular Virology and Gene Silencing Laboratory, Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic EngineeringFaisalabad, Pakistan.

Frontiers in Plant Science

|April 27, 2017

Summary

This summary is machine-generated.

Viral vectors offer a highly efficient method for delivering genome engineering (GE) reagents into plant cells. This approach overcomes limitations of traditional GE methods, enabling routine genetic modification in crops.

Keywords:

CRISPR/Cas9 DNA replicon Tobacco rattle virus (TRV)geminivirus genome engineering

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Last Updated: Mar 3, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

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Published on: February 27, 2021

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Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

Area of Science:

Plant biotechnology
Molecular biology
Genomics

Background:

Genome engineering (GE) enables precise DNA alteration in plants for desired traits.
Current GE methods like Agrobacterium or particle bombardment have limited cell transformation efficiency.
In planta GE approaches are slow, costly, and highly regulated.

Purpose of the Study:

To review recent advances in using viral vectors for plant genome engineering.
To discuss the limitations and future prospects of viral vector-mediated GE.
To highlight the need for efficient GE reagent delivery in crops.

Main Methods:

Utilizing autonomously replicating virus-based vectors for GE reagent delivery.
Employing DNA viruses (e.g., Bean yellow dwarf virus, Wheat dwarf virus, Cabbage leaf curl virus) and RNA viruses (e.g., Tobacco rattle virus).
Testing viral vectors in model plants (Nicotiana benthamiana) and crops (potato, tomato, rice, wheat).

Main Results:

Virus-based vectors demonstrate efficient delivery of GE reagents in plants.
Successful gene targeting frequencies observed in both model plants and important crop species.
Viral vectors show promise as an efficient alternative to conventional GE transformation methods.

Conclusions:

Virus-based vectors represent a significant advancement for plant genome engineering.
Overcoming delivery limitations is crucial for routine GE, especially in recalcitrant crops.
Further research into viral vector systems will accelerate crop improvement.