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Experimental sialodacryoadenitis virus infection in athymic CD-1 mice
D H Percy1, S J Bond, J I MacInnes
1Department of Pathology, Ontario Veterinary College, University of Guelph.
Summary
CD-1 nude mice showed minimal susceptibility to sialodacryoadenitis virus, with no significant lesions or antibody response detected. This suggests inadvertent exposure poses no threat to these mice in research settings.
Area of Science:
- Veterinary Virology
- Immunology
- Rodent Models
Background:
- Sialodacryoadenitis virus (SDAV) is a common murine coronavirus.
- Nude mice are frequently used in biomedical research due to their immunodeficiency.
- Understanding SDAV susceptibility in nude mice is crucial for experimental integrity.
Purpose of the Study:
- To determine the susceptibility of CD-1 nude mice to sialodacryoadenitis virus.
- To assess the potential for SDAV to cause disease or induce an immune response in this model.
Main Methods:
- Intranasal inoculation of young adult male CD-1 nude mice with SDAV.
- Necropsy and tissue examination (respiratory tract) at multiple time points post-inoculation.
- Virus isolation and serological testing (antibody detection) in mice.
- Comparative histopathology, immunofluorescence microscopy, and serology in Wistar rats.
Main Results:
- Minimal lesions were observed in the lower respiratory tract of infected mice; other tissues were unaffected.
- SDAV was isolated from the lower respiratory tract of mice at 6 and 8 days post-inoculation.
- No antiviral antibodies were detected in inoculated or control mice.
- Rats exhibited readily detectable virus-associated lesions and antibodies.
Conclusions:
- CD-1 nude mice demonstrate very low susceptibility to sialodacryoadenitis virus.
- Inadvertent exposure to SDAV is unlikely to pose a significant threat to CD-1 nude mouse colonies.
- The susceptibility of CD-1 nude mice to SDAV appears similar to that of euthymic CD-1 mice.