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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Charge detection mass spectrometry: weighing heavier things.

David Z Keifer1, Elizabeth E Pierson2, Martin F Jarrold3

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Charge detection mass spectrometry (CDMS) directly measures ion mass for single molecules. Recent advancements have revitalized CDMS, expanding its applications for high mass analytes.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Physical Chemistry

Background:

  • Charge detection mass spectrometry (CDMS) is a single-molecule technique.
  • It directly determines ion mass from mass-to-charge ratio and charge measurements.
  • CDMS is advantageous for analyzing high-mass and heterogeneous analytes, overcoming limitations of conventional mass spectrometry (MS).

Purpose of the Study:

  • To provide an overview of recent advancements in CDMS.
  • To describe the three main variants of CDMS.
  • To highlight recent applications of CDMS in various scientific fields.

Main Methods:

  • The article reviews technical developments in CDMS.
  • It discusses improvements in resolution and analytical capabilities.
  • The three primary CDMS variants are detailed.

Main Results:

  • CDMS has undergone significant technical improvements, enhancing resolution.
  • These advancements have broadened the scope of addressable problems.
  • CDMS is increasingly adopted for high molecular weight species analysis.

Conclusions:

  • Recent developments have revitalized CDMS, making it more mainstream.
  • The technique is crucial for analyzing complex, high-mass molecules.
  • CDMS offers powerful solutions where conventional MS methods fall short.