Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.8K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.8K
Micelles01:30

Micelles

51
Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
51
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

4.4K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
4.4K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.3K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.3K
Synthesis of Phosphatidylcholine in the ER Membrane01:27

Synthesis of Phosphatidylcholine in the ER Membrane

4.4K
The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...
4.4K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

4.3K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Addition to "Polarization-Multiplexed Dynamic Light Scattering: Characterizing Rotational Diffusion and Shape of Optically Anisotropic Particles".

Analytical chemistry·2026
Same author

Structural characterization of phosphatidylcholine lipid monolayers across the liquid-expanded/liquid-condensed phase transition.

Soft matter·2026
Same author

Polarization-Multiplexed Dynamic Light Scattering: Characterizing Rotational Diffusion and Shape of Optically Anisotropic Particles.

Analytical chemistry·2026
Same author

The association between prescribed hormonal contraception and multiple sclerosis risk: a systematic review and meta-analysis.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

Fatigue prevalence, interactions with associated symptoms and longitudinal changes in those with multiple sclerosis: Insights from the TONiC study.

Journal of the neurological sciences·2026
Same author

Radiation-dose effects in correlative X-ray/cryo-electron microscopy of frozen-hydrated biological samples.

Acta crystallographica. Section D, Structural biology·2026

Related Experiment Video

Updated: Mar 3, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

14.2K

Vesicle Origami: Cuboid Phospholipid Vesicles Formed by Template-Free Self-Assembly.

Frederik Neuhaus1,2, Dennis Mueller1, Radu Tanasescu1

  • 1Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700, Fribourg, Switzerland.

Angewandte Chemie (International Ed. in English)
|April 27, 2017
PubMed
Summary

Researchers developed a novel 1,2-diamidophospholipid that self-assembles into cuboid structures, challenging the spherical norm for phospholipid vesicles and revealing new insights into membrane physics.

Keywords:
cuboid vesiclesmembrane tensionphospholipidsself-assemblyvesicle origami

More Related Videos

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface
06:28

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Published on: May 1, 2020

4.1K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

3.0K

Related Experiment Videos

Last Updated: Mar 3, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

14.2K
Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface
06:28

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Published on: May 1, 2020

4.1K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

3.0K

Area of Science:

  • Supramolecular Chemistry
  • Materials Science
  • Biophysics

Background:

  • Phospholipid liposomes are typically spherical due to surface tension minimization.
  • Understanding phospholipid interactions is key to designing novel self-assembled structures.
  • Current models may overestimate lateral surface pressure in bilayer membranes.

Purpose of the Study:

  • To investigate novel phospholipid structures beyond spherical vesicles.
  • To explore the self-assembly behavior of a 1,2-diamidophospholipid.
  • To re-evaluate the physical principles governing phospholipid bilayer formation.

Main Methods:

  • Synthesis and characterization of a novel 1,2-diamidophospholipid.
  • Analysis of self-assembly into non-spherical structures using microscopy and biophysical techniques.
  • Measurement of lateral surface pressure within the assembled membranes.

Main Results:

  • A 1,2-diamidophospholipid was synthesized that self-assembles into a cuboid structure.
  • Intermolecular hydrogen bonding resulted in tight subgel packing and maximized flat surfaces.
  • Lateral surface pressure was found to be significantly lower than previously assumed values.

Conclusions:

  • Novel cuboid phospholipid structures can be formed, expanding the design space for self-assembled materials.
  • Hydrogen bonding plays a critical role in dictating the geometry of phospholipid assemblies.
  • The study challenges established assumptions regarding lateral surface pressure in bilayer membranes.