Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

4.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.1K
Lineage Commitment01:21

Lineage Commitment

4.5K
Commitment is the  process whereby stem cells:
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pig Kidney Xenotransplantation: Immune Insights From a Landmark Human Case.

American journal of kidney diseases : the official journal of the National Kidney Foundation·2026
Same author

Generation of Regulatory Dendritic Cells From Living Donor Liver Transplant Perfusates.

Transplantation·2026
Same author

Sustained function and composite immunity of a 61-day pig-to-human decedent thymokidney xenotransplant.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Single-cell RNA sequencing of healthy and diseased rat temporomandibular joint condyle cartilage.

PloS one·2026
Same author

BLIMP1 shapes germinal center B cell clonal diversity by gating chromatin accessibility during light-to-dark zone transition.

Nature immunology·2026
Same author

Donor-derived regulatory dendritic cell infusion and early immunosuppressive drug withdrawal in living-donor liver transplantation: a phase I/IIa trial.

Nature communications·2026

Related Experiment Video

Updated: Mar 3, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

59.0K

Phenotype, function, and differentiation potential of human monocyte subsets.

Lisa B Boyette1, Camila Macedo1, Kevin Hadi1

  • 1Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.

Plos One
|April 27, 2017
PubMed
Summary
This summary is machine-generated.

Human monocyte subsets (classical, intermediate, non-classical) show distinct immune functions. Classical monocytes differentiate into dendritic cells, while all subsets form macrophages, with non-classical monocytes producing interferon-alpha.

More Related Videos

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

7.6K
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

19.6K

Related Experiment Videos

Last Updated: Mar 3, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

59.0K
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

7.6K
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

19.6K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Human monocytes comprise distinct classical, intermediate, and non-classical subsets.
  • Their functional heterogeneity and differentiation potential remain incompletely understood.

Purpose of the Study:

  • To comprehensively characterize the functional properties and differentiation capabilities of human monocyte subsets.
  • To investigate their roles in immune responses and cytokine secretion.

Main Methods:

  • Phenotyping of peripheral blood monocyte subsets from healthy individuals.
  • Functional assays including cytokine secretion in response to TLR agonists.
  • Differentiation studies towards dendritic cells and macrophages.
  • High-speed cell sorting for subset isolation.

Main Results:

  • Monocyte subset frequencies are stable and tightly regulated.
  • Distinct cytokine secretion profiles (TNFα, IL-6, IL-1β, IFN-α) were observed across subsets in response to TLR stimulation.
  • Classical monocytes efficiently differentiated into monocyte-derived dendritic cells (mo-DCs).
  • All monocyte subsets differentiated into macrophages (M1/M2), exhibiting enhanced phagocytosis and cytokine production.
  • Non-classical monocytes were identified as a significant source of interferon-alpha (IFN-α).

Conclusions:

  • Classical monocytes are the primary precursors for mo-DCs.
  • All human monocyte subsets possess the capacity to differentiate into macrophages.
  • Monocytes, particularly the non-classical subset, contribute to type I interferon production during viral infections.