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Updated: Mar 3, 2026

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
Published on: November 10, 2017
[Expression of Death Associated Protein Kinase 1 in Chronic Lymphocytic Leukemia]
Guan-Ting Zhang1, Xiao-Jing Liu1, Lin Lu1
1Department of Clinical Laboratory Examination, The Affiliated Hospital of Henan Province Academy of Traditional Chinese Medicine, Zhengzhou 450004, Henan Province, China.
Objective:
To explore the expression of death-associated protein kinase 1(DAPK1) in chronic lymphocytic leukemia(CLL).
Methods:
The DAPK1 expression was studied by means of MEC1 cells and B lymphocytes from blood samples of the patients with CLL. The quantitative detection of mRNA and Western blot were used to detecte the expression of DAPK1 and autophagy-related genes at both mRNA and protein levels.
Results:
mRNA quantitative detection and Western blot displayed that the DAPK1 expression in the patients with CLL was silenced. So, the expression of DAPK1 and autophagy related genes in MEC1 cells was not significantly different, no matter the cells were treated with or without INF-γ.
Conclusion:
Scilencing of DAPK1 expression in CLL results in abnormality of autophagy behavior, thus leading to the occurence of disease.
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