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Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique.

Anastasia M Yunusova1, Veniamin S Fishman1,2, Gennady V Vasiliev3

  • 1Laboratory of Developmental Genetics, Institute of Cytology and Genetics, Novosibirsk 630090, Russia.

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|April 28, 2017
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Summary
This summary is machine-generated.

Cellular reprogramming to pluripotency is more deterministic than previously thought. A study using genetic barcoding revealed that reprogramming success is a heritable trait, suggesting pre-established potential within cell lineages.

Keywords:
cell fate decisionscellular barcodinginduced pluripotent stem cellsreprogramming to pluripotency

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Area of Science:

  • Stem cell biology
  • Cellular reprogramming
  • Epigenetics

Background:

  • Somatic cell reprogramming to pluripotency is inefficient, with only a few cells successfully reprogrammed.
  • Previous studies relied on population averages or limited single-cell live imaging to assess reprogramming potential.

Purpose of the Study:

  • To investigate the heritability of reprogramming potential in somatic cells.
  • To determine if reprogramming success is a pre-established trait within cell lineages.

Main Methods:

  • Application of lentiviral genetic barcoding to track related cells.
  • High-throughput sequencing of barcodes from reprogrammed cells.
  • Development of a computational model to estimate synchronous reprogramming probability.

Main Results:

  • Identification of a significant number of barcodes from related cells among successfully reprogrammed cells.
  • A computational model estimated a 10-30% probability of synchronous reprogramming in sister cells.
  • Reprogramming success appears to be a heritable trait maintained through cell division.

Conclusions:

  • Cellular reprogramming success is not entirely random but is pre-established in certain cells.
  • Reprogramming potential is a heritable trait that can be passed down through cell division.
  • The reprogramming process exhibits deterministic characteristics at the cell lineage level.