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Updated: Mar 3, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Clinical Evidence for the Microbiome in Inflammatory Diseases
Ann E Slingerland1, Zaker Schwabkey2, Diana H Wiesnoski2
1Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Abstract:
Clinical evidence is accumulating for a role of the microbiome in contributing to or modulating severity of inflammatory diseases. These studies can be organized by various organ systems involved, as well as type of study approach utilized, whether investigators compared the microbiome of cases versus controls, followed patients longitudinally, or intervened with antibiotics, prebiotics, or bacterial introduction. In this review, we summarize the clinical evidence supporting the microbiome as an important mechanism in the onset and maintenance of inflammation.
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