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Plasmids in coryneform bacteria of human origin
1Department of Microbiology, Institute of Dermatology, London, UK.
The Journal of Applied Bacteriology
|June 1, 1988
Summary
Plasmids were studied in human-sourced coryneform bacteria. Many strains contained diverse plasmids, and some antibiotic resistance was linked to plasmid loss, highlighting their varied roles.
Area of Science:
- Microbiology
- Molecular Biology
Background:
- Coryneform bacteria are found in human environments.
- Plasmids are extrachromosomal DNA elements that can confer traits like antibiotic resistance.
Purpose of the Study:
- To investigate the presence and diversity of plasmids in human-isolated coryneform bacteria.
- To explore the potential link between plasmids and antibiotic resistance in these bacteria.
Main Methods:
- Isolation and characterization of coryneform bacteria from human sources.
- Plasmid DNA extraction and analysis using endonuclease restriction digestion.
- Assessment of antibiotic resistance patterns and correlation with plasmid presence/loss.
Main Results:
- 89 plasmids were identified across 70 of 269 analyzed strains.
- Plasmids exhibited variations in size and structure.
- Antibiotic resistance was successfully cured in 6 out of 20 strains upon plasmid loss.
Conclusions:
- Human-isolated coryneform bacteria harbor a diverse array of plasmids.
- Plasmids play a significant role in conferring antibiotic resistance in these bacteria.
- The structural diversity of plasmids suggests varied functions and evolutionary pathways.