Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence that Lactobacillus casei insertion element ISL1 has a narrow host range.

M Shimizu-Kadota1, J L Flickinger, B M Chassy

  • 1Yakult Central Institute for Microbiological Research, Tokyo, Japan.

Journal of Bacteriology
|October 1, 1988
PubMed
Summary

The ISL1 insertion element from Lactobacillus casei has a very limited host range. DNA hybridization revealed ISL1 sequences in only a few L. casei strains, often on lactose plasmids.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for plasmid-associated lactose metabolism inLactobacillus casei subsp.casei.

Current microbiology·2013
Same author

A method to maintain introduced DNA sequences stably and safely on the bacterial chromosome: application of prophage integration and subsequent designed excision.

Journal of biotechnology·2001
Same author

Insertion of bacteriophage phiFSW into the chromosome of Lactobacillus casei strain Shirota (S-1): characterization of the attachment sites and the integrase gene.

Gene·2000
Same author

Thermostable alpha-galactosidase from Thermotoga neapolitana: cloning, sequencing and expression.

FEMS microbiology letters·1998
Same author

Copy number and location of insertion sequences ISS1 and IS981 in lactococci and several other lactic acid bacteria.

Journal of dairy science·1993
Same author

Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.

Nucleic acids research·1992

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • The insertion element ISL1 was initially identified in Lactobacillus casei S-1.
  • Understanding the distribution and host range of mobile genetic elements is crucial for bacterial evolution and adaptation.

Purpose of the Study:

  • To investigate the host range and prevalence of the ISL1 insertion element.
  • To determine if ISL1 is common among Lactobacillus casei strains and other related bacteria.

Main Methods:

  • DNA-DNA hybridization using Southern transfers.
  • A cloned internal fragment of ISL1 served as the molecular probe.
  • Screening of various Lactobacillus casei strains, other Lactobacillus species, and different bacterial genera.

Related Experiment Videos

Main Results:

  • ISL1 sequences were detected in only 3 out of 19 examined Lactobacillus casei strains.
  • In two positive L. casei strains, ISL1 sequences were located on lactose plasmids.
  • No homologous sequences to ISL1 were found in 14 other Lactobacillus strains or 15 strains from other bacterial genera.

Conclusions:

  • The ISL1 insertion element exhibits an extremely restricted host range, primarily within specific Lactobacillus casei populations.
  • ISL1's presence on lactose plasmids suggests a potential role in horizontal gene transfer within this limited host environment.