Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Colloidal gold immunostaining on deplasticized ultra-thin sections.

H Mar1, T N Wight

  • 1Department of Pathology, University of Washington, Seattle 98195.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The pharmacokinetics and dosing of oral 4-methylumbelliferone for inhibition of hyaluronan synthesis in mice.

Clinical and experimental immunology·2016
Same author

Differences in the distribution of versican, decorin, and biglycan in atherosclerotic human coronary arteries.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Tissue integrity signals communicated by high-molecular weight hyaluronan and the resolution of inflammation.

Immunologic research·2014
Same author

Differential effects of exogenous and endogenous hyaluronan on contraction and strength of collagen gels.

Acta biomaterialia·2008
Same author

Fenofibrate modifies human vascular smooth muscle proteoglycans and reduces lipoprotein binding.

Diabetologia·2004
Same author

Thiazolidinediones reduce the LDL binding affinity of non-human primate vascular cell proteoglycans.

Diabetologia·2004
Same journal

Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Aquaporin-4 Localization in Terminal Glial Cells of Human Mechanosensory Corpuscles: An Immunohistochemical Study of Penile Tissues.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
See all related articles

This study introduces a novel method for localizing tissue antigens on ultra-thin sections. The technique enhances antigen visibility and preserves tissue integrity, improving immunostaining specificity and reducing background noise.

Area of Science:

  • Immunohistochemistry
  • Electron Microscopy
  • Cell Biology

Background:

  • Embedding plastic can mask tissue antigens, hindering ultrastructural localization.
  • Unsupported thin sections are prone to drying artifacts during immunostaining.

Purpose of the Study:

  • To develop a method for effective ultrastructural localization of tissue antigens.
  • To improve antigen accessibility and preserve tissue integrity during immunostaining.

Main Methods:

  • Deplasticizing ultra-thin sections on-grid.
  • Incubating with primary antiserum and colloidal gold-conjugated immunoglobulin.
  • Re-embedding sections in dilute Epon after immunostaining.

Main Results:

Related Experiment Videos

  • Successfully localized previously masked tissue antigens.
  • Prevented drying artifacts by re-embedding the plastic matrix.
  • Achieved optimal preservation of tissue components.
  • Demonstrated enhanced immunostaining specificity with low background.

    • Conclusions:

    • The developed method allows for effective ultrastructural antigen localization.
    • This technique is beneficial for low-epitope-density samples and improves immunostaining quality.