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Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in
Vladan Bajić1, Biljana Spremo-Potparević2, Lada Živković2
1Institute of Nuclear Sciences Vinča, University of Belgrade, P.O. Box 522, Serbia.
Colloids and Surfaces. B, Biointerfaces
|April 28, 2017
Summary
Bare titanium dioxide nanoparticles (TiO2 NPs) exhibit stronger antigenotoxic effects than those modified with ascorbic acid (AA). The study found minimal differences in antigenotoxic and antioxidative properties between free and bound AA on TiO2 NPs.
Area of Science:
- Materials Science
- Nanotechnology
- Biochemistry
Background:
- Titanium dioxide nanoparticles (TiO2 NPs) are increasingly studied for their biological properties.
- Ascorbic acid (AA) is a well-known antioxidant.
- Investigating the synergistic or antagonistic effects of combining TiO2 NPs with AA is crucial for understanding their potential applications.
Purpose of the Study:
- To compare the antigenotoxic and antioxidative properties of surface-modified TiO2 NPs with ascorbic acid (AA) against their individual components.
- To characterize the charge transfer (CT) complex formed between TiO2 NPs and AA.
- To evaluate the in vitro antigenotoxic and antioxidant activities of bare TiO2 NPs, free AA, and AA-modified TiO2 NPs.
Main Methods:
- Synthesis of TiO2 NPs via acidic hydrolysis of TiCl4.
- Characterization using transmission electron microscopy (TEM) and X-ray diffraction (XRD).
- Formation and characterization of the charge transfer (CT) complex using UV-Vis spectroscopy, Job's method, Banesi-Hildebrand analysis, and FTIR.
- In vitro evaluation of antigenotoxicity using the comet assay in leukocytes.
- In vitro assessment of antioxidant properties via total antioxidant status (TAS) and total oxidant status (TOS) assays.
Main Results:
- Bare TiO2 NPs demonstrated more significant antigenotoxic effects compared to AA-modified TiO2 NPs and free AA.
- Charge transfer complex formation between TiO2 NPs and AA was confirmed.
- No substantial differences in antigenotoxic or antioxidative properties were observed between free AA and AA bound to TiO2 NPs within the tested concentrations.
Conclusions:
- Bare TiO2 NPs possess notable antigenotoxic potential.
- Surface modification of TiO2 NPs with AA does not significantly enhance or diminish the inherent properties of AA within the studied range.
- Surface-modified TiO2 NPs with AA hold promise for applications where maintaining beneficial activities is desired.
