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Updated: Mar 3, 2026
Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
Published on: September 14, 2018
Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
Maximillian T W Lee1, Antoine Maruani1, Daniel A Richards1
1Department of Chemistry , University College London , 20 Gordon Street , London , WC1H 0AJ , UK . Email: v.chudasama@ucl.ac.uk ; Tel: +44 (0)207 679 2077.
Abstract:
The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxidative conditions has hitherto not been achieved. The use of native antibodies has several advantages in terms of cost, practicality, accessibility, time and overall efficiency. Herein we present a novel, reliable method of furnishing antibody conjugates with a loading of two modules starting from a native antibody scaffold.
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