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Improving Cardiac Action Potential Measurements: 2D and 3D Cell Culture.

Neil J Daily1, Yue Yin1, Pinar Kemanli1

  • 1InvivoSciences, Inc., Madison, WI 53719, USA.

Journal of Bioengineering & Biomedical Science
|April 29, 2017
PubMed
Summary

Developing new assays to measure cardiac action potential is key for drug discovery and toxicity testing. This study presents an improved, cost-effective method using a fast plate reader and dyes for better signal detection in cardiomyocytes.

Keywords:
3DAction potentialCardiomyocyteTissueiPSC

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Area of Science:

  • Cardiovascular research
  • Stem cell biology
  • Assay development

Background:

  • Accurate measurement of cardiac action potential is vital for drug discovery and cardiotoxicity assessment.
  • Existing high-throughput methods utilize induced pluripotent stem cell (iPSC)-derived cardiomyocytes in 2D and 3D cultures.

Purpose of the Study:

  • To describe an improved, cost-effective method for assessing cardiac action potential.
  • To enhance signal detection for high-throughput screening in cardiac research.

Main Methods:

  • Utilized an ultra-fast, cost-effective plate reader.
  • Employed commercially available fluorescent dyes for action potential measurement.
  • Applied the method to induced pluripotent stem cell-derived cardiomyocytes.

Main Results:

  • Achieved dramatically improved detection of fluorescence signals.
  • Demonstrated a more efficient method for cardiac action potential assessment.
  • Enabled enhanced throughput for drug discovery and cardiotoxicity screening.

Conclusions:

  • The improved method offers a significant advancement for cardiac action potential assays.
  • This technique facilitates higher throughput in cardiac drug discovery and cardiotoxicity evaluation.
  • Paves the way for more advanced, high-throughput screening platforms in cardiovascular research.